Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants

Enzymes in living cells are highly dynamic but at the same time regularly confined for achieving efficient metabolism. Inspired by this phenomenon, we have prepared a novel biocatalytic membrane with high enzyme activity and stability tuning confinement strength of to enzyme, which was achieved via modifying support layer polymeric nanofiltration (NF) reversely filtrating enzyme. A mussel-inspired coating used modify interior NF enhance charge steric effects on thus stabilizing little increment mass transfer resistance substrate products (only 20% permeability loss loading 1.34 mg/cm2). suitable could delay leakage endow certain mobility reaction. Thus, obtained exhibited negligible decline BPA removal efficiency 7 reuse cycles (<3.5%) or 36 h continuous operation (<1%) flow through mode, resulting long-term adequate micropollutant removal. For first time, defined calculated quantify membrane, be optimize microenvironment immobilization predict performance membrane. This work concluded that rationally regulating periodic back-flushing redistribution enzymes achieve stable water